Spermidine trihydrochloride (Cas No. 334-50-9)

Description:
 

Spermidine is an endogenous polyamine. It is formed from putrescine by spermidine synthase. It is formed from putrescine by spermidine synthase.

Source:

Spermidine is found in fresh green pepper, wheat germ, cauliflower, broccoli, mushrooms, and a variety of cheeses. Even higher amounts are found in soybean products such as natto, shitake mushrooms, amaranth grain and durian. Some of these ingredients are particularly common in a Mediterranean diet, which may explain why some believe eating like the Spanish can prolong your life.

Uses:

Spermidine can be used in electroporation while transferring the DNA into the cell under the electrical impulse. May be used for purification of DNA-binding proteins. Spermidine is also used, along with calcium chloride, for precipitating DNA onto microprojectiles for bombardment with a gene gun.

Application:

Spermidine serves as a precursor of spermine and essential for both normal and neoplastic tissue growth. It was first detected in human sperm, but occurs widely in nature. It is essential in both normal and neoplastic tissue growth. Spermidine has a role in cell growth processes and the formation and interconversion of spermidine in mammalian cells has been reported. It has been studied in the regulation of tRNA methyltransferase activity and stimulates T4 polynucleotide kinase activity.

Definition:

Spermidine is a triamine that is the 1,5,10-triaza derivative of decane. It is a polyamine that is routinely included in restriction enzyme digestions to improve the cleavage efficacy of the DNA. Spermidine counteracts the inhibitory effects of contaminants coisolated with DNA and consequently permits complete digestion of the DNA at lower enzyme concentrations.

Preparation:

Raw materials:

Tetrahydrofuran-->Lithium Aluminum Hydride-->Sodium hydride-->Di-tert-butyl dicarbonate-->1,4-DIAMINOBUTANE-->3-BROMOPROPIONITRILE-->3-aminopropanal-->Spermine-->N-Ethyl-1,3-propanediamine-->3-(Benzylamino)propylamine-->Benzylamine